Pelin Sahlén

Associate professor, KTH

Key publications

Sahlén P, Spalinskas R, Asad S, Mahapatra KD, Höjer P, Anil A, Eisfeldt J, Srivastava A, Nikamo P, Mukherjee A, Kim KH, Bergman O, Ståhle M, Sonkoly E, Pivarcsi A, Wahlgren CF, Nordenskjöld M, Taylan F, Bradley M, Tapia-Páez I.
Chromatin Interactions in Differentiating Keratinocytes Reveal Novel Atopic Dermatitis and Psoriasis-Associated Genes. 
J Allergy Clin Immunol. 2020 Oct 15;. doi: 10.1016/j.jaci.2020.09.035. [Epub ahead of print] 

Pradhananga S, Spalinskas R, Poujade FA, Eriksson P, Sahlén P. 
Promoter anchored interaction landscape of THP-1 macrophages captures early immune response processes. 
Cell Immunol. 2020 Sep;355:104148. doi: 10.1016/j.cellimm.2020.104148. 

Cavalli M, Diamanti K, Pan G, Spalinskas R, Kumar C, Deshmukh AS, Mann M, Sahlén P, Komorowski J, Wadelius C.
A Multi-Omics Approach to Liver Diseases: Integration of Single Nuclei Transcriptomics with Proteomics and HiCap Bulk Data in Human Liver.
OMICS. 2020 Apr;24(4):180-194. doi: 10.1089/omi.2019.0215.

Åkerborg Ö, Spalinskas R, Pradhananga S, Anil A, Höjer P, Poujade FA, Folkersen L, Eriksson PP, Sahlén P.
High-Resolution Regulatory Maps Connect Vascular Risk Variants to Disease-Related Pathways.
Circ Genom Precis Med. 2019 Mar;12(3):e002353. doi: 10.1161/CIRCGEN.118.002353.

Zhang W, Chronis C, Chen X, Zhang H, Spalinskas R, Pardo M, Chen L, Wu G, Zhu Z, Yu Y, Yu L, Choudhary J, Nichols J, Parast MM, Greber B, Sahlén P, Plath K.
The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation.
Cell Stem Cell. 2019 Jan 3;24(1):138-152.e8. doi: 10.1016/j.stem.2018.12.001.

Anil A, Spalinskas R, Åkerborg Ö, Sahlén P.
HiCapTools: a software suite for probe design and proximity detection for targeted chromosome conformation capture applications.
Bioinformatics. 2018 Feb 15;34(4):675-677. doi: 10.1093/bioinformatics/btx625.

Sahlén P, Abdullayev I, Ramsköld D, Matskova L, Rilakovic N, Lötstedt B, Albert TJ, Lundeberg J, Sandberg R.
Genome-wide mapping of promoter-anchored interactions with close to single-enhancer resolution.
Genome Biol. 2015 Aug 3;16:156. doi: 10.1186/s13059-015-0727-9.

Pelin Sahlén

Research Interests

Promoters are central players in regulating expression levels of genes. However, it is through their interactions with distal acting enhancers, that they achieve specific patterns of expression in different cells, tissues and developmental stages.  In general, regulatory mutations carry lower burdens for the fitness of individuals than those of protein-coding mutations. Indeed, most of the variants associated with complex diseases are non-coding, and a large fraction of them (77%) are located within enhancers (Maurano MT et al, 2012). Therefore, it is of paramount importance to locate their target genes to discover their functional contribution to disease. 

We developed Capture Hi-C (HiCap) by combining Hi-C (4-cutter) and sequence capture methodologies to only capture interactions of regions of interest such as promoters (Sahlén P et al 2015). Recently, we applied Capture Hi-C in vascular and skin inflammatory disease context and discovered novel genes and processes potentially contributing to disease onset and progress through mapping risk variants to their target genes in relevant tissues. 

Currently our group is developing novel methodologies for the detection of promoter-enhancer interactions at the single cell level. We investigate the role of rare enhancer variants in human disease context. Moreover, we are mapping promoter-enhancer interactions in various dog and wolf tissues to establish the contribution of functional non-coding to domestication and adaptation of the dog from its wolf ancestors.

Group members

Jörg Bachmann, post-doc
Hassan Bassereh, post-doc
Artemy Zhigulev, PhD student
Robert William Baber, PhD student
Sailendra Pradhananga, PhD student

Contact information

Last updated: 2023-07-28

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