One of the biggest challenges in biology is to understand how apparently identical cells respond differently to the same stimulus. We use state of the art genomic technologies to study the regulatory mechanism leading to the appearance of divergent gene expression programs in clonal populations of cells.
To reach that aim, our group combines experimental and computational work to develop novel genome-wide techniques to study eukaryotic transcription. We are specially interested in the case of drug-tolerant cancer persister cells that, although genetically sensitive to a drug, do not respond to it. To deliver an integrated view of the mechanisms driving their appearance, as well as to refine our knowledge of the basic process of gene expression, we study both budding yeast and human cell lines at three levels of the gene expression process: epigenetic status, transcript isoform usage and post-transcriptional mRNA regulation.
- Pelechano V*, Wei W*, and Steinmetz LM. Widespread co-translational RNA decay reveals ribosome dynamics. Cell 2015 Jun 4;161(6):1400-12.
- Chabbert CD*, Adjalley SH*, Klaus B, Fritsch ES, Gupta I, Pelechano V#, and Steinmetz LM#. High-throughput ChIP-Seq for large-scale chromatin studies. Mol Syst Biol. 2015 Jan 12;11(1):777.
- Gupta I, Clauder-Münster S, Klaus B, Järvelin AI, Aiyar RS, Benes V, Huber W, Pelechano V# and Steinmetz LM#. Alternative polyadenylation diversifies post-transcriptional regulation through selective RNA-protein interactions. Mol Syst Biol. 2014 Feb 25;10(2):719.
- Pelechano V*, Wei W*, Steinmetz LM. Extensive transcriptional heterogeneity revealed by isoform profiling. Nature. 2013 May 2;497(7447):127-3
- Wilkening S*, Pelechano V*, Järvelin AI*, Tekkedil MM, Anders S, Benes V, Steinmetz LM. An efficient method for genome-wide polyadenylation site mapping and RNA quantification. Nucleic Acids Res. 2013 Mar 1;41(5):e65