Affinity Proteomics

Twitter: @plasmaprofiling

The Unit of Affinity Proteomics offers a long-standing expertise in the analysis of human body fluids, cells, and tissue lysates. We also provide analysis of protein-protein interactions and activation in situ or in tissues. Our services aim to support researchers and clinicians in the fields of translational and personalized medicine providing access to an exquisite infrastructure to apply cutting edge immunoassays technologies for multiplexed protein quantification.

With our expertise, we support biomarker studies from discovery to validation, in a large number of samples and a variety of human conditions. Our services include all aspects such as  study design, selection of antibodies, data processing and we also develop alternative assays tailored for the specific needs of the user’s projects.

Do you want to learn more about our technologies? Do you have an exciting project idea?

CONTACT US to discuss the optimal solutions for you!

Eatris

The Affinity Proteomics-Stockholm is now part of the Biomarker platform within the European infrastructure for translational medicine (EATRIS). EATRIS is a non-profit European Research Infrastructure Consortium (ERIC) which aims to facilitates collaboration among researchers, physicians, and developers and supports the establishment of multidisciplinary projects in the field of personalized medicine.

plasma profiling unit @eatris.eu

News

• Olink Explore HT, for the analysis of 5400+ proteins in only 2 µl sample, is now available at the Uppsala node
• In 2023 the Stockholm’s lab as been reconstructed and now is qualified as BSL 2
• A new system for high-throughput processing of volumetric dried blood spots is now available (PA496,Capitainer®)
• Acquisition of the Quanterix SPX Imaging and Analysis System Instrument for ultrasensitive chemokine/cytokines quantification

Services

Highly multiplexed and high-throughput protein profiling of human body fluids, cells tissue and extra vesicles lysates

• Proximity Extension Assays (Certified service provider for Olink Proteins AB)
  Target 96, Target 48, Flex and Focus (Stockholm and Uppsala)
  Explore HT 5400+ and Explore 384-3072 (Uppsala, contact: explorelab@scilifelab.uu.se)
• Multiplexed sandwich immunoassay (Commercial kits for Luminex System up to 40 targets) (Stockholm)

Advanced protein quantification in body fluids and cell/tissue lysates

• Automated microfluidic immunoassays (ProteinSimple) (Stockholm)
• Ultrasensitive immunoassays (Quanterix®) (Stockholm)
• MesoScale’s ECL products (Meso Scale Discovery) (Uppsala)
• Development of novel quantitative immunoassays (Homebrew Luminex and Quanterix® systems ) (Stockholm)
• Customized proximity extension (PEA) and proximity ligation assays (PLA) with dual and triple antibody recognition (Uppsala)

Protein analysis in fixed cells, tissue sections and extracellular vesicles

• Commercial in situ PLA assays (Navinci Diagnostics) (Uppsala)
• Development of customized PLA assays to detect proteins interactions and/or post-translational modifications
  in tissue (imaging), cells in suspension and extracellular vesicles(flow-cytometry) (Uppsala)
• Application of PLA sensitivity and specificity of Western blot (Uppsala)

Consultation for immunoassays

• Selection of antibodies targets (access to 50000 antibodies from the Human Protein Atlas (HPA)) (Stockholm)
• Antibody validation (Stockholm)
• Multiplexed assessment of optimal antibody pairs (Luminex System) (Stockholm)

Experimental design and data analysis

• Study design (Stockholm and Uppsala)
• Processing and analysis of affinity proteomics data (Stockholm and Uppsala)
• Statistical and bioinformatics analysis pipeline (Stockholm and Uppsala)

Applications

• High-throughput, exploratory protein profiling of human body fluids
• Biomarkers discovery and validation in large sample cohorts
• Robust protein quantification for candidate biomarkers
• analysis of proteins interactions and/or post-translational modifications in cells and tissues
• Development of novel immunoassays concepts
• Antibody validation and selectivity analysis

Equipment

Reagents

• 50,000 antibodies from the Human Protein Atlas project

Analytical equipment

• 2x FLEXMAP 3D, Luminex®
• LX200, Luminex®
• MAGPIX, Luminex®
• ELLA, ProteinSimple®,
• Biomark HD, Fluidigm®
• SIMOA SR-X, Quanterix®
• SIMOA SP-X Imaging and Analysis System, Quanterix®
• MESO QuickPlex SQ 120MM imager, Mesoscale®
• MESO SECTOR S 600MM imager, Mesoscale®
• CytoFLEX S Violet-Blue-Yellow Green-Red (V-B-Y-R)
• Olink Explore (SPT Mosquito and Dragonfly dispensers, EpMotion liquid handler, NovaSeq 6000 sequencer, Hamilton Stars, NovaSeq X, ProFlex PCRs) (ExploreLab, in collaboration with NGI Uppsala)
• Olink Signature Q100
• QuantStudio6 Flex Real-Time PCR Systems with 96 and 384 interchangeable blocks. Applied Biosystems
• C1 Single-Cell Auto Prep system, Fluidigm®
• Epifluorescence microscopes: fully automated stages on Zeiss Axio Imager Z2 and Leica DMi8
• Molecular Devices MetaExpress automated scanning microscope.
  

Samples handling equipment

• FLUENT, Tecan
• 2x EVO150, Tecan
• SELMA, CyBio®
• PA496 Pre-analytical Automation for qDBS analysis, Capitainer®

Magnetic bead handling equipment

• KingFisher Flex, Thermo
• EL406 Washer Dispenser, Biotek
• 405 LS and TS, Biotek
• Simoa Microplate Washer system, Quanterix®

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Using our services

In order to get in touch with us and use our units – please fill out the unit Project form:

Project Form

This information will allow us to understand how we can be of best help for you. We will then come back to you with if and how our units can be of best help for your project.