Integrated Microscopy Technologies (IMT)

Contact

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Julia Fernandez-Rodriguez (CAT support)

julia.fernandez-rodriguez@cci.sahlgrenska.gu.se

Rafael Camacho (CAT support)

rafael.camacho@gu.se

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Sara Henriksson (FIB-SEM support)

sara.henriksson@umu.se

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Ana Agostinho (ExM, SIM, STED, SMLM, MINFLUX imaging support)

ana.agostinho@scilifelab.se

Stefan Wennmalm (FCS, FCCS, FRET-FCS, STED-FCS, MINFLUX tracking support)

stefan.wennmalm@scilifelab.se

Steven Edwards (Clearing, Expansion, Light-sheet & Lattice light-sheet support)

steven.edwards@scilifelab.se

Recent user publications

The publications in this database are the result of research conducted at the units of SciLifeLab – both in user projects and technology development.

Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure

E. Sezgin,

A red fluorescent lifeact marker to study actin morphology in podocytes.

E. Wiesner, J. Binz-Lotter, SE. Tröder, (…), T. Benzing, R. Wedlich-Söldner, MJ. Hackl,

Sci Rep15 (1) 12386

Cell membranes sustain phospholipid imbalance via cholesterol asymmetry.

M. Doktorova, JL. Symons, X. Zhang, (…), E. Lyman, KR. Levental, I. Levental,

Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures

M. Hawgood, B. Urién González, A. Agostinho, (…), J. Bartek, H. Blom, B. Lemmens,

Personnel

ALM Staff

Hjalmar Brismar, Platform Scientific Director ALM/CMI

Hans Blom, Head of ALM unit (Super-resolution Microscopy support)

Ana Agostinho, Application Expert Super-resolution Microscopy support

Stefan Wennmalm, Manager Single Molecule Dynamics support

Steven Edwards, Manager Light-Sheet & Lattice Light-Sheet Microscopy support

SciLifeLab / Services / Our infrastructure / Integrated Microscopy Technologies (IMT)

Integrated Microscopy Technologies (IMT)

Advanced Light Microscopy (ALM), Correlative Array Tomography (CAT) and Focused Ion Beam Scanning EM (FIB-SEM)

LINK to CAT services at Center for Cellular Imaging in Gothenburg

LINK to FIB-SEM services at Umeå Center for Electron Microscopy

–> APPLY FOR IMT SUPPORT here LINK.

Advanced Light Microscopy

The mission of the Advanced Light Microscopy (ALM) national unit is to give support with superresolution fluorescence microscopy for nanoscale biological visualisation (SIM, STED, SMLM, ExM, MINFLUX). In addition the unit support single molecule measurement and analysis with fluorescence correlation spectroscopy (FCS/FCCS/FRET-FCS), and combined with superresolution for nanoscale dynamical studies (STED-FCS, MINFLUX tracking). Moreover, support with light-sheet fluorescence microscopy (LSFM) allow users to image live and/or optically cleared larger samples at unprecedented volumetric speed with low phototoxity. Single cell ultra-fast volumetric imaging of biological processes at high-resolution is provided by lattice light-sheet microscopy (LLSM).

Services

Support is done to all stages of the project including (pre)planning, sample optimization, fluorescent probe selection, image acquisition, and initial post-acquisition image processing.

Super-resolution microscopy (SIM, STED, SMLM, ExM, MINFLUX) – nanoscale cellular imaging of fixed or living samples, and cleared/expanded tissue.
Fluorescence Correlation Spectroscopy – single molecule spectroscopy measurement and analysis to evaluate interaction, aggregation, mobility, dynamics.
Light-sheet microscopy – fast, volumetric imaging of organoids, model organisms and cleared/expanded tissues.
Lattice light-sheet microscopy – fast high-resolution volumetric imaging of cellular processes in mammalian and plant cells.

Transfer of unique knowledge to individual researchers are supported nationally, including organization of workshops and courses in advanced fluorescence imaging and spectroscopy applications and method developments.

Unit video link: https://youtu.be/mqx49azmqtY

Methods

SIM, STED, SMLM, ExM, MINFLUX

Investigation of molecular architecture of sub-cellular entities.
Organization and localization in cell biology with sub-diffraction resolution.

FCS/FCCS/FRET-FCS/STED-FCS

Analysis of receptor-receptor, ligand-receptor, protein-protein, protein-peptide and protein-vesicle interactions in solutions and in living cells
Aggregation and affinity analysis at single molecule concentration levels in solutions