Spatial mass spectrometry is a label-free method that allows for the visualization and quantitative analysis of the distribution of targeted or untargeted chemical species directly in tissue sections. We utilize advanced mass spectrometry imaging (MSI) instruments and ionization methods, i.e., matrix-assisted laser desorption ionization (MALDI) and desorption electrospray ionization (DESI). The speed, sensitivity, and molecular specificity of the mass spectrometers enable the direct and simultaneous imaging of hundreds to thousands of molecules (in each pixel). The Spatial Mass Spectrometry unit provides a service for the imaging of drugs, their metabolites and endogenous biomolecules such as neurotransmitter systems, lipids, peptides, and small proteins directly in tissue sections at near-cellular lateral resolution.
We perform everything from project design, sample preparation and imaging of tissue sections to optimize the project results by using bioinformatic tools. All projects are unique and might require individual method developments to different extents.
Proposed projects are assessed and prioritized according to the following model
Initial screen of project by unit
Project prioritization is based on
MSI enables simultaneous and label-free quantitative analysis of drugs and metabolites across multiple tissue types.
MSI provides an option for tissue classification at the molecular level and could thus be very useful as an alternative or complement to histology for the diagnosis and characterization of toxicological findings.
MSI visualizes and quantifies the brain distribution of drugs with varying blood-brain barrier permeability, determines the blood-brain barrier permeability and defines the drug distribution in very small brain structures.
MSI provides a strategy for simultaneously imaging, identifying and quantitating the absolute concentrations of multiple neurotransmitters and neurotransmitter precursors and metabolites directly in brain tissue sections.
MSI has the ability to detect and characterize tumour cells and their environment in a spatial context and can be a complement to aid pathologists and clinicians.
Mass spectrometry instruments
The two solariX MALDI instruments allow for the mapping of the distribution of drugs and metabolites, neurotransmitters, neuropeptides and lipids. The FTICR instruments offer ultrahigh mass resolving power that maximizes the number of analytes detected. They require tissue sections to be placed on conductive glass slides.
The ultrafleXtreme instruments can be used for a broad variety of MALDI-MSI applications and can analyse samples at near cellular lateral resolution (10 µm). Requires tissue sections to be placed on conductive glass slides.
The MALDI SYNAPT G2-Si is suited for analysis of tissue sections placed on regular microscope glass slides (non-conductive). Lateral resolution of 20 µm.
Analysis with the DESI Xevo TQ-XS instrument allows targeted imaging of small molecules on regular microscope glass slides (non-conductive) at a lateral resolution of 20 µm.
Matrix application and tissue cutting instruments
Data analysis and bioinformatics tools
Please send us an email with questions, suggestions or project proposals using the contact emails provided. We will get back to you shortly after to schedule a meeting for further discussions.