Spatial Proteomics
Our unit provides service in highly multiplexed immunofluorescence (up to 40 markers) of tissue sections. Since 2017 we offer service using the Phenocycler platform (previosuly known as CODEX)). The PhenoCycler technology is compatible for fresh frozen (FF) and paraffin embedded (FFPE) tissue sections from mouse and human. We offer the complete list of pre-conjugated antibodies available from Akoya Biosciences and the list of available antibodies can be found here. In addition, we can perform in house custom antibody conjugation for essential targets of your project. Using the PhenoCycler platform we can also perform up to 8 plex staining using the Opal chemistry and Phenocode signature panels, developed for more clinical assays.
We also offer multiplexing using the COMET instrument from Lunaphore-Biotechne. This is based on sequential IF using primary off the shelf antibodies. Depending on your need and specific project we will discuss what methods for multiplexing that is the most suitable for each project.
We also offer more standard immunofluorescence solutions and hematoxyin and eosin stainings. These protocols can be done in combination with the PhenoCycler or COMET protocols.
We have long time experience with developing custom assays to fit your project needs and we are happy to start exploring how we can drive your project forward with the help of highly multiplexed imaging!
Services
We perform everything from project design, sample preparation and imaging to give you as good results as possible.
Proposed projects are assessed and prioritized according to the following model
Initial screen of project by unit:
- Technical feasibility (yes/no)
- Capacity requested for the project (classification: small/medium/large)
- User ability to analyze the generated data
Project prioritization is based on:
- Scientific potential
- Significance of unit specific technique for project
- Supporting preliminary data
- Supports unit development (competence and techniques)
- Feasibility of time lines
Applications
- Cell type distribution and interactions in tissue samples
- Targeted protein marker expression across samples
- Combined protein and RNA expression within tissue sections (under imlementation)
Equipment
- PhenoCycler – enabling highly multiplexed imaging (>30) of proteins at single cell level in tissue sections
- PhenoImager – using Opal dyes to stain up to 7 markers + dapi in tissue sections
- COMET – enabling automated sequential immunofluroescence of 8-20 proteins
- Evos s1000 – enables imaging up to 8 proteins in tissue sections at higher throughput. Service under implementation
Using the unit
Please send us an email with questions, suggestions or project proposals using the contact emails below. We will get back to you shortly after to schedule a meeting for further discussions if applicable.