Our unit provide service in highly multiplexed immunofluorescence (up to 30 markers) of tissue sections using the CODEX platform (service area 2). The CODEX technology is compatible for fresh frozen (FF) and paraffin embedded (FFPE) tissue sections from mouse and human. We offer the complete list of pre-conjugated antibodies available from Akoya Biosciences and the list of available antibodies can be found here. In addition, we can perform in house custom antibody conjugation for essential targets of your project.
The unit also enables researchers to access the resources and expertise from the Human Protein Atlas (www.proteinatlas.org) and the primary aim is to do full service immunofluorescence projects at moderate to high-throughput level in cell lines. Based on the resource of more than 25,000 antibodies and a panel of >30 different human cell lines, we offer a unique possibility to explore hundreds of protein targets at subcellular level under various cellular states and conditions (service area 1).
We perform everything from project design, sample preparation and imaging to give you as good results as possible. We share infrastructure and resources with the Human Protein Atlas and this include a proteome wide library of antibodies that can be used in tailor made projects to answer specific biological questions. You are also welcome to use our microscopes for imaging after an introduction with one of the staff scientists.
Proposed projects are assessed and prioritized according to the following model
Initial screen of project by unit:
Project prioritization is based on:
Here are a few examples of previous projects:
Please send us an email with questions, suggestions or project proposals using the contact emails below. We will get back to you shortly after to schedule a meeting for further discussions if applicable.