Therapeutic Protein Engineering

Infrastructure Unit


Esmeralda Woestenenk

Recent user publications

The publications in this database are the result of research conducted at the units of SciLifeLab – both in user projects and technology development.


Per-Åke Nygren, Platform Scientific Director
Esmeralda Woestenenk, Head of Unit
Leif Dahllund
Juni Andrell
Ainhoa Moliner-Morro
Oskar Andersson
Pauliina Turunen

Therapeutic Protein Engineering

The Therapeutic Protein Engineering (TPE) unit provides recombinant proteins needed for biochemical screening and therapeutic applications as well as antigens for human antibody production using bacterial and mammalian expression systems. The unit also houses competence and machinery to characterize purified proteins in detail, like binding, stability, and aggregation tendency.

The TPE unit also does phage display selection, characterisation and engineering of antibodies and antibody fragments. This work is shared with our sister unit in Lund: Display & Selection Technologies. More information can be found under that unit.


  • Delivery of pharmaceutical target proteins for screening and MOA studies including:
    • Construct design, cloning and expression analysis
    • Expression in E. coli, insect cells, transient and stable pool mammalian cells up to 20L scale
    • Purification and biophysical characterisation of proteins
  • Expression, purification and characterisation of biopharmaceutical candidates for Proof of Concept and MOA studies
  • qPCR expression analysis
  • Mammalian cells for screening
  • Thermal shift screens (DSF)
  • Support in biochemical screening assay design and setup


Watch our cells grow!
  • Fully equipped lab for protein expression and purification
  • ambr15 Microbioreactor
  • Light Cycler 480 II
  • Isothermal calorimeter
  • Biacore 8K
  • Liquid handling robot
  • Prometheus NT.48 with Backreflection Optics
  • Malvern DLS
  • Refeyn Mass Photometer TwoMP

Last updated: 2024-05-21

Content Responsible: Esmeralda Woestenenk(