Research Interest
Mitochondria are involved in a plethora of diseases. As every cell type has unique metabolic demands, the mitochondrial disease signature is disease-, tissue- and ultimately cell-type-specific. We study the normal working range of mitochondria and its tipping points into disease. This goes beyond understanding mitochondria as bioenergetic hubs and encompasses their various integrated functions throughout the cell.
Proteomics offers a powerful route to explore mitochondrial processes, particularly because many mitochondrial proteins are inaccurately captured by other omics approaches. We isolate target cells from intact tissue specimens from both model systems and human biopsies, and combine laser microdissection with mass spectrometry through Deep Visual Proteomics (DVP) to resolve proteomes at single-cell resolution while preserving tissue context. We actively develop the technology towards autonomous cell selection and more robust LC-MS workflows to enable high-throughput single-cell analysis.
Group Members
- Akos Vegvari
- Charu Sharma
- Florian Rosenberger
- Jessica Guedes
- Kathakali De
- Kilian Preussner
- Natasa Dorka Rácz
- Simon Wetzel