Integrated Microscopy Technologies (IMT)

Contact

IMT-GOTHENBURG (CAT & NanoSIMS support)

imt-gothenburg@scilifelab.se

IMT-UMEÅ (FIB-SEM support)

sara.henriksson@umu.se

IMT-STOCKHOLM (ALM support) – SIM, STED, SMLM, ExM, MINFLUX imaging

ana.agostinho@scilifelab.se

MOLECULE DYNAMICS – FCS, FCCS, FRET-FCS, STED-FCS, MINFLUX tracking

stefan.wennmalm@scilifelab.se

VOLUMETRIC IMAGING – Clearing, Light-sheet & Lattice light-sheet

steven.edwards@scilifelab.se

Recent user publications

The publications in this database are the result of research conducted at the units of SciLifeLab – both in user projects and technology development.

Engineered calcium-regulated affinity protein for efficient internalization and lysosomal toxin delivery.

M. Jönsson, M. Möller, L. Schierholz, (…), M. Wolf-Watz, H. Brismar, S. Hober,

Proc. Natl. Acad. Sci. U.S.A.122 (48) e2509081122

Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems

F. Ragaller, AM. Schneider, E. Sjule, (…), M. Dustin, A. Achour, E. Sezgin,

Prodan-based solvatochromic probes for polarity imaging of organelles

N. Aknine, P. Holban, F. Ragaller, (…), A. Cetinkaya, E. Sezgin, AS. Klymchenko,

Dual-color expansion microscopy of membrane proteins using bioorthogonal labelling

S. Edwards, B. Meineke, S. Bauer, H. Blom, S. Elsässer, H. Brismar,

Personnel

IMT-GOTHENBURG (CAT & NanoSIMS)
Julia Fernandez Rodriguez, Platform Scientific Director
Nhu Phan, Platform Scientific Director
Rafael Camacho, Head of Unit
Yanan Yang, Principal Research Engineer

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IMT-UMEÅ (FIB-SEM)
Linda Sandblad, Platform Scientific Director
Richard Lundmark, Platform Scientific Director
Sara Henriksson, Head of Unit

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IMT-STOCKHOLM (Advanced Light Microscopy)
Hjalmar Brismar, Platform Scientific Director
Hans Blom, Head of ALM unit (Super-resolution Microscopy support)
Ana Agostinho, Application Expert Super-resolution Microscopy support
Stefan Wennmalm, Manager Single Molecule Dynamics support
Steven Edwards, Manager Light-Sheet & Lattice Light-Sheet Microscopy support

SciLifeLab / Infrastructure / Our infrastructure / Integrated Microscopy Technologies (IMT)

Integrated Microscopy Technologies (IMT)

Follow links and information below to access technologies supported through the Integrated Microscopy Technologies (IMT) infrastructure unit, which include Correlative Array Tomography (CAT), Nanoscale Secondary Ion Mass Spectrometry (nanoSIMS), Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) and Advanced Light Microscopy (ALM).

–> APPLY FOR IMT SUPPORT here LINK.

IMT-Gothenburg: CAT & NanoSIMS

Correlative array tomography (CAT) combines microscopy modalities to explore the 3D cellular architecture of large samples in extremely fine structural and molecular detail by, for example, merging the molecular discrimination of light microscopy with the ultrastructural imaging of electron microscopy.

LINK to CAT services at University of Gothenburg.

Nanoscale secondary ion mass spectrometry (nanoSIMS) offers a secondary ion nanoprobe to carry out nanoscale projects, a unique technology in the Nordic region, to address questions in materials, geological, marine, environmental, medical and life science fields.

LINK to NanoSIMS services at University of Gothenburg.

IMT-Umeå: FIB-SEM

Focused ion beam scanning electron microscopy (FIB-SEM) reveals subsurface structural detail by making precise cuts with a FIB, then imaging the exposed surface with a high-resolution SEM.

LINK to FIB-SEM services at Umeå University.

IMT-Stockholm: Advanced Light Microscopy

The mission of the IMT Stockholm unit / Advanced Light Microscopy (ALM) facility is to give support with nanoscale biological visualisation. In addition, we support molecular dynamics analysis by fluorescence correlation spectroscopy methods, and superresolution additions for nanoscale dynamics. Moreover, light-sheet fluorescence microscopy allow users to image live and/or optically cleared larger samples at unprecedented volumetric speed with low phototoxity. Single cell ultra-fast volumetric imaging of biological processes at high-resolution is further provided by live lattice light-sheet microscopy.

Services (ALM)

Support is done to all stages of the project which can include (pre)planning, sample optimization, fluorescent probe selection, image acquisition, and initial post-acquisition image processing.

Super-resolution microscopy – nanoscale imaging of fixed, cleared or expanded cells and tissue.
Fluorescence Correlation Spectroscopy – single molecule dynamics in-vitro or in-situ.
MINFLUX tracking – ultra-fast nanoscale dynamics of proteins and lipids in cell membranes.
Light-sheet microscopy – volumetric imaging of organoids, model organisms and cleared tissues.
Lattice light-sheet microscopy – fast high-resolution volumetric imaging of cellular processes.

Transfer of unique knowledge to individual researchers are supported nationally, including organization of workshops and courses in advanced fluorescence imaging and spectroscopy applications and method developments.

IMT Stockholm (ALM) overview support: https://youtu.be/mqx49azmqtY

Methods (ALM)

SIM/STED/SMLM/ExM/MINFLUX imaging

Investigation of molecular architecture of sub-cellular entities.
Organization and localization in cell biology with sub-diffraction resolution.

FCS/FCCS/FRET-FCS/STED-FCS/MINFLUX tracking

Analysis of receptor-receptor, ligand-receptor, protein-protein, protein-peptide and protein-vesicle interactions in solutions and in living cells
Aggregation and affinity analysis at single molecule concentration levels in solutions
Molecular nanoscale tracking and dynamic analysis of proteins and lipids