High Throughput Genome Engineering – SciLifeLab Infrastructure Unit in Solna is seeking to recruit a staff scientist

Karolinska Institutet

Application deadline

February 14, 2021

SciLifeLab Infrastructure Unit in Solna is seeking to recruit a staff scientist

Do you want to contribute to top quality medical research? 

About High Throughput Genome Engineering

HTGE is a national SciLifeLab unit based at the Department of Medical Biochemistry and Biophysics in Biomedicum at Solna campus. As the foremost academic CRISPR competence centre in the Nordics, our mission is to develop and implement high-throughput CRISPR methodologies and support the Swedish research community in the design, execution, and interpretation of CRISPR-based functional genomic experiments. Examples include genome-wide, pooled CRISPR-based loss-and gain-of-function screens, screening with customised sgRNA libraries, small screens with single cell transcriptomic readout, targeted mutagenesis screens for studying drug-target interactions, CRISPR screens with in situ guide sequencing, and others. We work exclusively in cell culture systems, and perform all high-throughput experiments in pooled format. We collaborate closely with other SciLifeLab units, such as the Chemical Biology Consortium Sweden (CBCS), NGI, Eukaryotic Single Cell Genomics, and others. Our second arm, Karolinska Genome Engineering, focuses on precision genome engineering in cells and has common pipelines with Karolinska’s iPSC core unit and the Karolinska Centre for Transgene Technologies.

Your mission.

As part of the team, your primary task will be to design and execute high-throughput CRISPR-Cas projects for unit clients. In addition, you will have an important contribution in incorporating the latest cutting-edge technologies into HTGE’s service portfolio. You will have frequent customer contact, and will commonly be involved in several projects simultaneously.

Your profile.

Candidates are required to be fluent in English, hold a PhD degree in a relevant subject and need extensive experience in mammalian cell culture. Postdoctoral experience is advantageous, as is documented prior experience in CRISPR-Cas9-based pooled genetic screening or similar technologies. Familiarity with lentiviral tools, molecular cloning techniques, as well as skills in flow cytometry and cell sorting are highly relevant, as are programming skills in R and/or Python. We work in a fast-paced environment, and excellent time management and research documentation skills, as well as the ability to multi-task are essential. Constant contact with our collaborators and unit clients requires good interpersonal skills, and the ability to communicate clearly and effectively. Personal suitability is of great importance when evaluating the applicants.

What do we offer?

HTGE provides a friendly and stimulating work-environment located in one of Europe’s most modern research buildings. The projects we run come from all areas of molecular cell biology, providing insights into many different research fields. Personal development through courses and conferences is explicitly encouraged. The position will be for one year in the first instance, with the possibility of extension by another year

Karolinska Institutet is one of the world’s leading medical universities. Our vision is to pursue the development of knowledge about life and to promote better health for all. At Karolinska Institutet, we conduct innovative medical research and provide the largest range of biomedical education in Sweden. Karolinska Institutet is a state university, which entitles employees to several benefits such as extended holiday and a generous occupational pension. Employees also have free access to our modern gym and receive reimbursements for medical care.

Location: Solna


Welcome to apply at the latest 210214

Type of employmentTemporary position longer than 6 months
Contract typeFull time
First day of employmentUpon agreement
SalaryMonthly salary
Number of positions1
Working hours100%
CountyStockholms län
Reference numberSTÖD 2-203/2021
ContactBernhard Schmierer, bernhard.schmierer@ki.se
Union representativeVenus Azhary, OFR, 0707-124 127
Last application date14.Feb.2021 11:59 PM CET

Last updated: 2021-01-25

Content Responsible: David Gotthold(david.gotthold@scilifelab.se)