Resercher in Cryo-EM
at the Department of biochemistry and biophysics. Closing date: 3 April 2022.
The Department is mainly located with the other Departments of Chemistry and Life Sciences in the Arrhenius Laboratories for Natural Sciences, which are situated in the northern part of the University Campus at Frescati. Presently more than 300 people are working at the Department of which about 110 are PhD students engaged in internationally highly recognized research covering a broad range of subjects. To read more visit: www.dbb.su.se.
We have one research position available using cryo-EM to study AAA+ ATPases and their regulation in bacteria and humans in the group of Dr. Marta Carroni, located at SciLifeLab. The candidate will work closely with members of the Cryo-EM Facility at SciLifeLab (Stockholm, Sweden) and will have access to two Titan Krios (equipped with Gatan Bioquantum-K3 cameras and CetaD for electron diffraction), one Talos Arctica (Falcon 3 and K2) and a powerful GPU computer cluster. SciLifeLab offers access to a number of other facilities such as super-resolution fluorescence microscopy, compound screening, NMR and structural mass spectrometry offering numerous possibilities to learn, explore cutting edge techniques, and to collaborate with different experts.
Project description
The research group aims to address the following questions. How do AAA+ protein unfoldases and proteases confer bacterial resistance? How protein regulators tune these AAA+ protein activities? More in general, how AAA+ chaperones interact with their client proteins? We aim at understanding the mechanism of action of AAA+ at the molecular level by combining mechanistic insights from structural analysis with functional studies in the cellular context.
Main responsibilities
The position involves mainly research. The candidate will also have to help in the supervision of a PhD student and will be involved in some teaching. The postdoctoral researcher is expected to take the lead on and be responsible for experimental design and laboratory work, as well as interpretation of data, data management, writing, and publishing, in collaboration with the project team.
Qualification requirements
Researchers are appointed primarily for purposes of research and must hold a Swedish doctoral degree or an equivalent degree from another country.
Assessment criteria
In the appointment process, special attention will be given to research skills. The candidate should have a PhD Biophysics, Chemistry, Molecular Biology or similar areas with sound experience in protein production, protein structure determination using cryo-EM single particle analysis and model building. Experience with cryo-electron tomography and sub-tomogram averaging or knowledge in X-ray crystallography, NMR spectroscopy and strucutre prediction is a plus. Experience with fluorescence imaging is also a strong merit. Knowledge of compound screening is advantageous. Strong communication skills in English, in both speech and writing are of the highest value. Great importance will be given to personal suitability.
Terms of employment
The position involves full-time employment for a maximum of two years, with the possibility of extension. Start date as per agreement.
Stockholm University strives to be a workplace free from discrimination and with equal opportunities for all.
Contact
Further information about the position can be obtained from Dr. Marta Carroni, telephone: +46 8 16 10 13, marta.carroni@scilifelab.se.
Union representatives
Ingrid Lander (Saco-S), telephone: +46 708 16 26 64, saco@saco.su.se, Alejandra Pizarro Carrasco (Fackförbundet ST/Lärarförbundet), telephone: +46 8 16 34 89, alejandra@st.su.se, and seko@seko.su.se (SEKO).
Application
Apply for the position at Stockholm University’s recruitment system. It is the responsibility of the applicant to ensure that the application is complete in accordance with the instructions in the job advertisement, and that it is submitted before the deadline.