Molecular Mechanism of RNA Splicing by the Spliceosome
October 21, 2025 @ 15:30 – 17:00 CEST
Seminar by Yigong Shi
Westlake University, Hangzhou, Zhejiang 310024, China
Host: David Drew, SciLifeLab
Splicing of the precursor messenger RNA (pre-mRNA) is an indispensable step in the information flow from DNA to protein in all eukaryotes. Research of the past four decades has led to accumulation of a large body of biochemical and genetic information on pre-mRNA splicing. Structural information is central to the elucidation of the molecular mechanism of pre-mRNA splicing by the spliceosome. In 2015, we reported the first atomic structure of an intact spliceosome from S. pombe at an average resolution of 3.6 Å. This structure reveals the overall organization and the conserved atomic features of an intact spliceosome, which turns out to be a protein-orchestrated metalloribozyme. Since then, atomic structures have been reported for nearly all major functional states of the spliceosome, allowing recapitulation of the entire splicing cycle and mechanistic understanding of RNA splicing. In this presentation, I will summarize these advances and discuss implications on disease treatment.
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