Emma Lundberg, Associate Professor (docent)
Cell Profiling facility
KTH Royal Institute of Technology
Our research is focused on spatial proteomics and cell biology. At the interface between bioimaging, proteomics and cell biology are fundamental aspects of human cell biology assessed using state-of-the-art microscopy in a systematic and proteome-centric manner. The aim is to understand how human proteins are distributed in time and space, how variations and deviations in localization can contribute to cell type specificity as well as disease.
Compartmentalization of biological processes is a fundamental principle of eukaryotic cells that enables multiple processes to occur in parallel. The compartment types are specialized for a particular cellular function and contain molecules for that purpose. Defects in compartment organisation or protein translocation underlie the cause of many forms of human disease. Despite a great deal of research, basic questions about the spatial organization of many proteins and biological processes remain unanswered.
A part of our work is the creation of the Subcellular Protein Atlas as part of the Human Protein Atlas project, where the subcellular localization of all human proteins is being determined.
We are currently running a Citizen Science project called “Project Discovery” for classification of protein patterns in microscope images, integrated into the massively multiplayer online Sci-Fi game Eve Online.
Martin Hjelmare, Lab manager
Lars Björk, Lab manager
Hammou Ait Blal, Facility project coordinator
Charlotte Stadler, Postdoc
Devin Sullivan, Postdoc
Peter Thul, Postdoc
Frida Danielsson, PhD-student
Diana Mahdessian, PhD-student
Mikaela Wiking, PhD-student
Anna Bäckström, Research engineer
Rutger Schutten, Research engineer
Christian Gnann, Research engineer
Casper Winsnes, Research engineer
Jenny Fall, Lab technician
- Uhlén M, Fagerberg L, Hallström BM, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Å, Kampf C, Sjöstedt E, Asplund A, Olsson I, Edlund K, Lundberg E, Navani S, Szigyarto CA, Odeberg J, Djureinovic D, Takanen JO, Hober S, Alm T, Edqvist PH, Berling H, Tegel H, Mulder J, Rockberg J, Nilsson P, Schwenk JM, Hamsten M, von Feilitzen K, Forsberg M, Persson L, Johansson F, Zwahlen M, von Heijne G, Nielsen J, Pontén F. Tissue based map of the human proteome. Science (2015)
- Danielsson F, Skogs M, Huss M, Rexhepaj E, O’Hurley G, Klevebring D, Pontén F, Gad AK, Uhlén M,Lundberg E. Majority of differentially expressed genes are down-regulated during malignant transformation in a four-stage model. PNAS (2013).
- Stadler, Rexhepaj, Singan, Murphy, Pepperkok, Uhlén, Simpson, Lundberg. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods (2013).
- Stadler, Hjelmare, Neumann, Jonasson, Pepperkok, Uhlén, Lundberg. Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy. J Proteomics (2012).
- Lundberg, Fagerberg, Klevebring, Matic, Geiger, Cox, Algenäs, Lundeberg, Mann, Uhlen. Defining the transcriptome and proteome in three functionally different human cell lines. Mol Syst Biol. (2010).