Biophysical Screening and Characterization

Infrastructure Unit

Recent user publications

The publications in this database are the result of research conducted at the units of SciLifeLab – both in user projects and technology development.


Helena Danielson, Platform Scientific Director

Sherry Mowbray, Platform Scientific Director

Annette Roos, Head of Unit

Helena Nordström

Eldar Abdurakhmanov


Biophysical Screening and Characterization

The unit provides in depth information on interactions. The data can range from simple qualitative analyses demonstrating that two molecules interact, to quantitative analyses that provide mechanistic, kinetic, thermodynamic, chemodynamic information as well as molecular details of complexes. The unit primarily aids projects in the Drug Discovery and Development Platform, but can also provide services to academic and external researchers.


  • Kinetic characterization of hit or lead compounds with the surface plasmon resonance (SPR) technology (Biacore).
  • KD values of biomolecular interactions in solution using the method microscale thermophoresis (MST).
  • Affinity ranking of compounds with SPR or MST.
  • Protein crystallization, structure determination by X-ray crystallography and structure analysis.
  • X-ray crystallography for visualizing the interactions between a protein and a ligand for in silico optimization of hit or lead compounds.
  • Fragment based drug discovery – screening with SPR and crystallizing target protein with hit molecules for structure based fragment growth.

To summarize, we can provide information for drug discovery and development projects relating to:

  • Validation of target functionality and reference ligands
  • How to prove target engagement
  • Definition of suitable kinetic profile of leads
  • Resolving mode-of-action and complex interaction mechanisms


  • Biacore T200.
  • Instruments for high-throughput crystallization such as a Mosquito® Crystal liquid handler, a Scorpion liquid handler and a Rigaku plate hotel.
  • Monolith NT. Automated (MST instrument from Nanotemper). Lable free and fluorescent red-blue detectors.

Using the unit

Please contact Annette Roos or go to the Drug Discovery and Development Platform page for more information

Mail address

Uppsala University 
Biophysical screening and characterization, 
Department of Pharmacy, 
Box 576
751 23 Uppsala, Sweden

Visitor address / Deliveries

Uppsala University
Biophysical screening and characterization, 
Department of Pharmacy, BMC
Husargatan 3
752 37 Uppsala, Sweden

Last updated: 2024-03-05

Content Responsible: Annette Roos(