The National Facility for Exposomics at SciLifeLab (Campus Solna) is coordinated by Stockholm University and the Department of Environmental Science (ACES). With expertise in small molecule mass spectrometry in human and environmental samples [1-4], we operate as infrastructure within the bigger SciLifeLab Metabolomics & Exposomics Platform.
The exposome was first described in 2005 to encompass all environmental exposures throughout the lifetime, and exposomic techniques continue to evolve with a purpose to better characterize what we are exposed to and how these exposures interact as risk factors for disease [5-7]. The current infrastructure is dedicated to study the chemical fraction of the exposome which is increasingly accessible by high resolution mass spectrometry [8, 9].
Applications
Our mission is to make state-of-the-art methods in high resolution chemical exposomics available to a wide user base of national and international researchers who wish to understand exposure and effects of complex small molecule chemical mixtures through the analysis of human, wildlife, and environmental samples. We continually adapt best available technologies and workflows to meet demands of our users for targeted and untargeted chemical exposomics in a range of sample types, including air and water, and a range of biofluids and tissues [1-4]. Our general working philosophy is that the data processing workflows should be achievable with open science tools to fully support data FAIRness, as demanded by the national research audience today.
We offer coverage of the chemical exposome. Flexible services are based on the research study hypotheses and user needs, including sample preparation for different human biofluids and environmental samples, and combination of different mass spectrometry analyses. We offer:
Comprehensive trace analysis by liquid (LC) or gas chromatography (GC) with high-resolution mass-spectrometry detection using Orbitrap technology.
Combined target and nontarget acquisition by data-dependent (DDA) and data-independent (DIA) MS/MS, with electrospray or atmospheric pressure chemical ionization
Sample preparation in ultratrace clean laboratory for a range of environmental and human samples (water, air, human plasma or urine, wildlife tissues)
In-line solid phase extraction for water or biofluids
Structural elucidation of unknown small molecules at trace concentration in complex samples
Data processing with open science software and FAIR workflows
Multivariate data analysis
Access to service follows SciLifeLab guidelines, and is open on equal terms to all Swedish academic users, as well as international users, and researchers within the healthcare, governmental agencies, and private sector. Highest priority is given to Swedish academic users. Project are evaluated and prioritized based on scientific and societal impact, and technical feasibility. Large-scale initiatives that address grand societal challenges within life science are favored for their impact.
Equipment
Positive pressure high efficiency particulate (HEPA)-filtered clean laboratory.
High-resolution mass spectrometers:
UHPLC 480 Exploris Orbitrap, LC-HRMS
UHPLC 120 Exploris Orbitrap, LC-HRMS
UHPLC Q Exactive HF-X Orbitrap, LC-HRMS
GC Q Exactive Orbitrap, GC-HRMS
Data processing with open science software (e.g. MS-DIAL) to sustain FAIR Data Principles GC Q Exactive Orbitrap, GC-HRMS (Autosampler)UHPLC Q Exactive HF-X Orbitrap, LC-HRMSUHPLC Q Exactive HF-X Orbitrap, LC-HRMS (on-line SPE) GC Q Exactive Orbitrap, GC-HRMS UHPLC 480 Exploris Orbitrap, LC-HRMS Positive pressure high efficiency particulate (HEPA)-filtered clean laboratory
At SciLifeLab, we use necessary cookies to ensure that the website works properly for you. We also want to use cookies for web analytics to help us improve the website. You can change your cookie settings at any time. How we use cookies
Functional
Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes.The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.
