Research Specialist to CRISPR Functional Genomics – SciLifeLab National Facility
Description of the subject area
CFG (https://www.scilifelab.se/units/crispr-functional-genomics/) is a national SciLifeLab facility based at the Department of Medical Biochemistry and Biophysics in Biomedicum at Solna campus. As the foremost academic competence centre for CRISPR in the Nordics, our mission is to develop, implement and offer high-throughput CRISPR technologies to support the Swedish research community in the design, execution, and interpretation of CRISPR-based functional genomic experiments. Examples include genome-wide, pooled CRISPR-based loss-and gain-of-function screens, screening with customised sgRNA libraries, small screens with single cell transcriptomic readout, targeted mutagenesis screens for studying drug-target interactions, CRISPR screens with in situ guide sequencing, and others. We work exclusively in cell culture systems, and perform all high-throughput experiments in pooled format. We collaborate closely with other SciLifeLab facilities, such as the Chemical Biology Consortium Sweden (CBCS), Chemical Proteomics, NGI, Eukaryotic Single Cell Genomics, and others. Our second arm, Karolinska Genome Engineering (https://ki.se/en/mbb/karolinska-genome-engineering), focuses on precision genome engineering in cells and has common pipelines with Karolinska’s iPSC core facility and the Karolinska Centre for Transgene Technologies.
As part of our current team of five, your primary task will be to design and execute functional genomic CRISPR screening projects for and together with CFG clients. You will also drive technology development and incorporate the latest cutting-edge technologies into CFG’s service portfolio. You will have frequent customer contact and will handle several projects simultaneously.
Candidates are required to hold a PhD degree in a relevant subject and need extensive experience in mammalian cell culture. Documented prior experience in CRISPR-Cas9-based pooled genetic screening or similar technologies is advantageous. A good grasp of standard molecular biology techniques such as cloning, PCR, qPCR, etc. is expected. Familiarity with lentiviral tools, flow cytometry and cell sorting are highly relevant, as are bioinformatic skills. We work in a fast-paced environment, and excellent time management and research documentation skills, as well as the ability to multi-task are essential. Frequent contact with our collaborators and clients demands good interpersonal skills, and the ability to communicate clearly and effectively. Fluency in English in speaking and writing is required.
After an overall assessment of the expertise and merits of the applicants in relation to the subject area, Karolinska Institutet will determine which of them has the best potential to contribute to a positive development of the activities at KI.
What do we offer?
A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world’s leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is a state university, which entitles to several benefits such as extended holiday and generous occupational pension. Employees also have access to our modern gym for free and receive reimbursements for medical care.
CFG provides a friendly and stimulating work-environment located in one of Europe’s most modern research buildings. The projects we run come from all areas of molecular cell biology, providing insights into many different research fields. Personal development through courses and conferences is explicitly encouraged. The position will be for one year in the first instance, with the possibility of extension by another year.