The CRISPR Functional Genomics unit (formerly High Throughput Genome Engineering, HTGE) provides affordable access to high throughput functional genomic screens using the CRISPR/Cas9 system in cell lines. Pooled CRISPR/Cas9 screening enables parallel interrogation of thousands to tens of thousands of genes for involvement in biological processes of interest. CFG provides access to verified lentiviral CRISPR guide libraries for whole genome and targeted loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition, CRISPR activation).
Our service portfolio includes CRISPR gene perturbation followed by single cell RNASeq (together with Eukaryotic Single Cell Genomics (ESCG) and we are developing base-editing approaches for targeted mutagenesis screens to characterize protein-protein or protein-drug interactions.
Barcoded guide libraries.
CFG is open to the entire Swedish and international research community. Please contact bernhard.schmiererATki.se to schedule a free consultation meeting.
Our local branch, Karolinska Genome Engineering (KGE), performs precision edits in cell lines, such as knock-out, knock-in, deletions, point mutations, etc. KGE collaborate with the Karolinska Center for Transgene Technologies (KCTT) to offer CRISPR technology in mouse, and has an active pipeline for precision editing of iPSCs with the iPSC core facility at Karolinska Institutet.
Your project is out of the box? All the better, we would love to hear from you!
Shipping and Mailing Address
171 65 Solna
171 65 Solna