Decoding the development of the human spinal cord by multi-omics
May 11 @ 14:00 – 15:30 CEST
Decoding the development of the human spinal cord by multi-omics
Xiaofei Li and Erik Sundström
HDCA Seminar Series 2022 | Spinal cord
The human spinal cord has highly diverse cell types, which are generated during development, governed by a series of temporal and spatial events for proper spinal cord assembly and function. However, the molecular regulation of cell fate specification in the human developing spinal cord remains largely unknown. Here, by performing single-cell RNA sequencing, spatial transcriptomics and in situ sequencing, we integrate the datasets and provide a comprehensive single-cell and spatial transcriptomic atlas of the developing human spinal cord of the first trimester, from postconceptional week 5 to 12. We observe that all major cell populations in human adult spinal cord have been specified within the first trimester with abundant cell states, and identify the key genes associated with cell fate choices and their spatial expression. Unexpectedly, we find that during early cell fate specification, many human neural progenitor cells have lost their self-renewal capacity. Further the the expression of gliogensis genes is much higher in human compared to the correspondent rodents at the early stage, suggesting earlier gliogenesis in human. Finally, we demonstrate how to use our developmental map for molecular cues that direct sequential cellular differentiation and define cells and locations associated to both normal development and spinal cord diseases.
Please register also if you want to participate in the digital broadcast. The Zoom link will be sent out through the registration system.
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Human developmental cell atlas (HDCA) is the Swedish effort within the human cell atlas (HCA), one of the largest international scientific projects. The project aim is to create a comprehensive molecular atlas of human prenatal development at the molecular resolution using state-of-the-art multi-omic technologies such as single-cell RNA-seq, Spatial Transcriptomics, in situ sequencing in order to provide deeper insight into how variations and deviations contribute to health and disease.
The HDCA Seminar Series 2022 invites you to join us on a journey of early human organogenesis of several organs at the single-cell and spatial resolution. Seminars will be held at SciLifeLab Gamma building on Wednesdays at 14:00 or 15:00 and also via Zoom. After each seminar, we will have up to 30 min round up discussions with the speaker on how you can contribute to the atlas!
Warm Welcome!
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