High Throughput Genome Engineering (HTGE)
The High Throughput Genome Engineering (HTGE) facility provides affordable access to high throughput functional genomic screens using the CRISPR/Cas9 system in cell lines. Pooled CRISPR/Cas9 screening enables parallel interrogation of thousands to tens of thousands of genes for involvement in biological processes of interest. HTGE provides access to verified lentiviral CRISPR guide libraries for whole genome and more targeted loss- and gain of function studies (CRISPR knock-out, CRISPR inhibition, CRISPR activation). We also offer generation of stable Cas9-expressing lines in users’ cells of interest. CROPSeq and CRISPR-X approaches are in development. HTGE aims to remain competitive and we adopt new screening strategies in collaboration with interested clients. HTGE supports the Swedish and international research community with CRISPR screening projects from planning to data analysis.
Your project is out of the box? All the better, we would love to hear from you!
Latest innovation available at HTGE:
CRISPR/Cas9 Screening Using Unique Molecular Identifiers. Bernhard Schmierer, Sandeep K. Botla, Jilin Zhang, Mikko Turunen, Teemu Kivioja, Jussi Taipale. Molecular Systems Biology 2017. PDF. Full text.
The precision and accuracy of CRISPR/Cas9 screens is dramatically improved by the incorporation of inert, random sequence labels (RSLs) into CRISPR guides.
- Compared to the conventional method, inclusion of RSLs generates considerably more information at an identical experimental scale and enables data analysis by simple statistics.
- RSL‐based analysis requires fewer cells per guide to reach a set statistical power. This is important if cell numbers are limiting, such as in very large, genome‐wide screens and/or screens in primary cells.
- RSLs can be used as Unique Molecular Identifiers (UMIs), allowing tracking of single cells and their progeny throughout a pooled screen.
- Cas9-expressing cell line generation
- High throughput pooled CRISPR screens from screen design to gene hit list
- Experienced support for screen design
- Lentiviral CRISPR guide libraries produced and provided
- Custom libraries created according to user specifications
- Library transduction performed
- Next generation sequencing library prepared
- Next generation sequence using HiSeq2500 at NGI Stockholm
- Data analysis: hit list generated
- Screens in cell lines, stem cells or primary cells
- Determine essential genes in specific cell types
- Screen for drug resistance/sensitivity genes
- Find novel genes/pathways involved in differentiation
- Map novel genes/pathways regulating a reporter gene
- Pooled screening is extremely versatile; any phenotype that allows cell separation can be queried!