Erik A.L. Biessen, Maastricht University, the Netherlands In this paper, Biessen et al. introduce a novel approach that integrates 15-plex multispectral immunofluorescent microscopy with mass spectrometry imaging to reveal murine atherosclerotic plaque myeloid heterogeneity. It maps the phenotypes’ tissue localization while allowing their further histological characterization and correlations with their cellular and molecular microenvironmental contexts. […]